The total flavone contents in variety of Citrus limon and in Citrus aurantium var. amara were the highest, which were 2.74% and 2.91 respectively.
A pathogen of black spot of citrus limon is primary identified by some observations and fungi cultivations of slices of ground infested leaves or fruits.
Some 80% of Costa Rica's exports and imports, and a chunk of Nicaragua's, pass along a single-carriageway road and a modest wharf at the run-down Caribbean port of limon.