Determination of related substances in ampicillin sodium and sulbactam sodium for injection by RP-HPLC gradient elution method
Objective:To study on chromatography fingerprint(CFP) of Huangqi herbs. Method: HPLC was used on a Hypersil ODS column with water-acetonitriles gradient elution.
Conclusions the reversed phase high performance liquid chromatography gradient elution method is simple and accurate for the determination of the related substances in allopurinol sodium injection.
Objective to establish a reversed phase high performance liquid chromatography gradient elution method for determination of the related substances in allopurinol sodium for injection.
Methods:(1) To separate stilbene glycoside by using silica gel column chromatography with gradient elution consisting of ethyl acetate and petroleum ether.
Zorbax ODS column and gradient elution of acetonitrile acetic acid solution as mobile phase were employed to determine the content of syringin and rutin in 11 species of Saussurea by RP_HPLC.
