The initiation of DNA replication in mammalian cells is a highly coordinated process that ensures duplication of the genome only once per cell division cycle.
HSP90 is an important member of HSP protein family. HSP90 usually constitutively expressed in most mammalian cell types and can be further induced by heat shock and other stresses.
The formal genetics of mammalian cells has been dependent on breeding experiment with whole organisms.
Most mammalian cells contain multiple species of DNA dependent DNA polymerases.
Until now, however, no one had applied the visualization technique to observing a single gene in mammalian cells.
The chief problem with previous methods for visualizing transcription in mammalian cells is that these require researchers to blast cells with hundreds of copies of the specially tagged gene.
