Methods The restriction endonuclease and T4 DNA ligase were used to construct the vector plasmid.
The products of endonuclease digestion were run on 8% non-denaturing polyacrylamide gel electrophoresis and detected by silver staining.
Methods The restriction endonuclease and T4 DNA ligase were used to construct the vector plasmid.
The products of endonuclease digestion were run on 8% non-denaturing polyacrylamide gel electrophoresis and detected by silver staining.